Structure-guided drug development for treatment of rifampin-resistant TB
X-ray crystallographic study of the mechanism of transcription
Cryo-EM to capture structures of macromoles in transcription process
X-ray crystal structure of RNA polymerase and DksA/ppGpp complex
Bacteriophage N4 RNA polymerase
Welcome to the Murakami Lab at Penn State University
Our research interests are centered on understanding the mechanism of gene expression by the enzyme RNA polymerase. We apply X-ray crystallography and cryo-electron microscopy techniques to reveal three-dimensional structures of bacterial, archaeal and bacteriophage RNA polymerases for elucidating the mechanism of RNA transcription and its regulation.
August 14, 2018: A review paper for introducing the structure and function of E. coli RNA polymerase is published in EcoSal Plus.
July 10, 2018: A paper reporting the crystal structures of the N4 phage RNA polymerase II is published in J. Biol. Chem.
March 28, 2018: A paper reporting the Cryo-EM structure of E. coli RNA polymerase open complex is published in J. Biol. Chem.
November 09, 2017: Yeonoh Shin will give a seminar (12:05 pm, 107 Wartik Laboratory) “Structural and Biochemical Studies of Bacterial RNA Polymerase Engaged in Reiterative Transcription during Transcript Initiation at pyrG and pyrBI Promoters”
October 26, 2017: Catherine Sutherland will give a seminar (12:05 pm, 107 Wartik Laboratory) “Rifampicin Resistance in Mycobacterium tuberculosis: Investigating Fitness Cost and Subsequent Compensation”
October 16, 2017: Our study, the crystal structure of reiterative transcription complex, is featured on CHESS website.
June 25-30, 2017: Vadim, Zuhaib, Anoop, Catherine, Yeonoh and Katsu attended and presented their works at the FASEB summer research conference “Mechanism and Regulation of Prokaryotic Transcription” at Saxtons River, Vermont. More…